Finger printing using RM primer

[narottam dey]

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Respected sir,
       Thanking your for your valuable suggestion.
No I am not try to relate the RIL screening (with RM-559) with that of germplasm screeening with RM-112.
What I want to tell  is that whenever I screen with a particular RM primer to RIL I can easily get the allelic combination in the RIL it may be homzygous or heterozygous for a particular locus related to that of RM primer.
I have no problem regarding it.
But regarding scrrening of germplasm I get hatdly polymorphism among accessions.
And it is now clear to me as u told me that though apparently in naked eye it seems to be non polymorphic there may be some polymorphism if I use made sizing through genotyping software.
But now my question is that as in case of SSR polymophism arise due to variation in repeat.  say in variety A the specificband  is of 124 bp.in case of Variety B it is of 126 and in C it is 128 and so on....then we can say that we can easily differentiate these variety by using that particular RM primer.
But does these difference give any reliable information for differetiate them as they are at a single locus and not a nuber of genomic region.
But for QTL mapping and marker development  they are fine.
For that the question arise in my mind is that is SSR scrrening for gemplasm is more powerful than that of RAPD.
With regards,
Narottam    

Narottam Dey.
Senior Research Fellow.
Dept Of Botany
Bose Institute.
93/1,APC road,
Kolkata-700009,(W.B)
India.
Ph-91-33-23679670 (R),23506619-ext-330(o)