<P>Respected sir,</P>
<P> Thanking your for your valuable suggestion.</P>
<P>No I am not try to relate the RIL screening (with RM-559) with that of germplasm screeening with RM-112.</P>
<P>What I want to tell is that whenever I screen with a particular RM primer to RIL I can easily get the allelic combination in the RIL it may be homzygous or heterozygous for a particular locus related to that of RM primer.</P>
<P>I have no problem regarding it.</P>
<P>But regarding scrrening of germplasm I get hatdly polymorphism among accessions.</P>
<P>And it is now clear to me as u told me that though apparently in naked eye it seems to be non polymorphic there may be some polymorphism if I use made sizing through genotyping software.</P>
<P>But now my question is that as in case of SSR polymophism arise due to variation in repeat. say in variety A the specificband is of 124 bp.in case of Variety B it is of 126 and in C it is 128 and so on....then we can say that we can easily differentiate these variety by using that particular RM primer.</P>
<P>But does these difference give any reliable information for differetiate them as they are at a single locus and not a nuber of genomic region.</P>
<P>But for QTL mapping and marker development they are fine.</P>
<P>For that the question arise in my mind is that is SSR scrrening for gemplasm is more powerful than that of RAPD.</P>
<P>With regards,</P>
<P>Narottam </P>
Narottam Dey.<br>
Senior Research Fellow.<br>
Dept Of Botany<br>
Bose Institute.<br>
93/1,APC road,<br>
Kolkata-700009,(W.B)<br>
India.<br>
Ph-91-33-23679670 (R),23506619-ext-330(o)