[Gramene] wheat rice comparison

[Somers, Daryl]

Hello Gramene

 

Please provide me with the process and route through the Gramene site to
do the following.

 

Start with a Wheat EST accession number

Compare this (sequence) to the Rice genome sequence and

Finish with a wheat/rice sequence alignment showing the proposed wheat
intron/exon boundaries.

 

I want to develop PCR primers from the wheat coding sequence that likely
span an intron.

 

Please indicate which features etc need to be on/off.

 

Hope you can help.

 

Regard

 

 

 

Daryl J. Somers

Agriculture and Agri-Food Canada

Cereal Research Centre

195 Dafoe Road

Winnipeg, MB, Canada

R3T 2M9

phone: 204-984-4503

fax: 204-983-4604

 

SomersD at agr.gc.ca <mailto:SomersD at agr.gc.ca> 

 

 

 

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