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<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Hello Gramene</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Please provide me with the process and route through the Gramene
site to do the following.</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Start with a Wheat EST accession number</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Compare this (sequence) to the Rice genome sequence and</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Finish with a wheat/rice sequence alignment showing the proposed
wheat intron/exon boundaries.</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>I want to develop PCR primers from the wheat coding sequence
that likely span an intron.</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Please indicate which features etc need to be on/off.</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Hope you can help.</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Regard</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Daryl J. Somers</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Agriculture and </span></font><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Agri-Food</span></font><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'> </span></font><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Canada</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Cereal Research Centre</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>195 Dafoe Road</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Winnipeg</span></font><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>, </span></font><font size=2
face=Arial><span style='font-size:10.0pt;font-family:Arial'>MB</span></font><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>, </span></font><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Canada</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=FR-CA style='font-size:
10.0pt;font-family:Arial'>R3T 2M9</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=FR-CA style='font-size:
10.0pt;font-family:Arial'>phone: 204-984-4503</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=FR-CA style='font-size:
10.0pt;font-family:Arial'>fax: 204-983-4604</span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span lang=FR-CA
style='font-size:12.0pt'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><a href="mailto:SomersD@agr.gc.ca"><span lang=FR-CA>SomersD@agr.gc.ca</span></a></span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span lang=FR-CA
style='font-size:12.0pt'> </span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><img border=0 width=278 height=24
src="cid:image001.jpg@01C882C0.ACE416A0"
alt="Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada"></span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> </span></font></p>
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