rice and arabidopsis promoters
Gregory S. Brown
gregb at biotiquesystems.com
Tue Sep 19 09:27:26 EDT 2006
Stuart,
The MartView system in gramene can help with data extraction problems
like this. Start here: http://www.gramene.org/Multi/martview and
select the species dataset you need. Then the next page allows you
to restrict the dataset of extracted genes by many different
criteria. Since you are interested in all gene sequences simply
proceed on to the next page by hitting the "next" button again.
Notice on the right hand side the resulting number of gene models
from the selected dataset you are working with. For Arabidopsis
TAIR6 gene models there are 30,569 entries in the db. Now to extract
sequence data for the selected gene models you must select
"Sequences" from the "Select Attribute Page menu at the very top of
this page. Notice all the different types of sequences you can
choose that include where appropriate upstream and downstream
flanking regions. Input in the size of the flanking regions and then
select appropriate fasta header information to include in the
extracted sequence. Hit the export button to extract the information
you need.
There is a good help page for the MartView system. In fact there is
an excellent help section for most pages in gramene. Simply find the
red "Help Desk" button located generally on the upper right hand side
of most pages. Then look at the list of help topics on the left hand
side.
Hope this helps.
gb
Greg Brown
On Sep 18, 2006, at 8:22 AM, Stuart Meier wrote:
> To whom it may concern,
> I am a post-doctoral fellow at The University of the Western Cape in
> South Africa. I am trying to download promoter sequences for
> arabidopsis and rice (oryza sativa) from the gramene website without
> success. I require nucleotide sequences -3000 to +500, relative to
> the transcription start sie, for all genes in the genome. Could
> you be
> kind enough to inform me if this is possible your web site and provide
> some directions on how this could be done.
> your help will be greatly appreciated
> regards
> Dr Stuart Meier
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