PCR product with RM-473D

[narottam dey]

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Respected sir,
  Thanking you for your kind cooperation valuable suggestion.
Now I am trying to clarify the pronlem which I told u in last mail
My Ph.D work is QTL mapping (a mapping population F-9 of IR-72 X Pusa Basmati)and SSLP based fingerprinting of a collection of aromatic both indigenous and basmati and some HYV rice cultivars.
For that I have choosen some RM primers, out of which RM-47D is there. Here I am sending you a PAGE picture of PCR product with RM-473D and RM-505 along with the cutivars name.
In case od RM-473D the size of PCR poduct with RM-473D is coincide your suggestion   I think there is a minute difference in IR-72 and Pusa Basmati (the parent of my population). Can I use this marker as polymorphic marker for my population?
Another point is that in IR-72 there is an extra band of around 265 bp(? at picture ) can I consider this band for polymorphism screening in parental polymorphism screening as well as varietal polymprhism screening.
Similarly in case of PCR product with RM-505 I got polymorphism in main band but in addition to this band I also getting a polymorhic band of arround 489 bp.Should I consider this band.
With regards,
Narottam

Narottam Dey.
Senior Research Fellow.
Dept Of Botany
Bose Institute.
93/1,APC road,
Kolkata-700009,(W.B)
India.
Ph-91-33-23679670 (R),23506619-ext-330(o)
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