<P>Respected sir,</P>
<P> Thanking you for your kind cooperation valuable suggestion.</P>
<P>Now I am trying to clarify the pronlem which I told u in last mail</P>
<P>My Ph.D work is QTL mapping (a mapping population F-9 of IR-72 X Pusa Basmati)and SSLP based fingerprinting of a collection of aromatic both indigenous and basmati and some HYV rice cultivars.</P>
<P>For that I have choosen some RM primers, out of which RM-47D is there. Here I am sending you a PAGE picture of PCR product with RM-473D and RM-505 along with the cutivars name.</P>
<P>In case od RM-473D the size of PCR poduct with RM-473D is coincide your suggestion I think there is a minute difference in IR-72 and Pusa Basmati (the parent of my population). Can I use this marker as polymorphic marker for my population?</P>
<P>Another point is that in IR-72 there is an extra band of around 265 bp(<STRONG>? at picture </STRONG>) can I consider this band for polymorphism screening in parental polymorphism screening as well as varietal polymprhism screening.</P>
<P>Similarly in case of PCR product with RM-505 I got polymorphism in main band but in addition to this band I also getting a polymorhic band of arround 489 bp.Should I consider this band.</P>
<P>With regards,</P>
<P>Narottam</P>
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Narottam Dey.<br>
Senior Research Fellow.<br>
Dept Of Botany<br>
Bose Institute.<br>
93/1,APC road,<br>
Kolkata-700009,(W.B)<br>
India.<br>
Ph-91-33-23679670 (R),23506619-ext-330(o)