[Gmod-help] Databases and chromosome location searches

Nigel Wilson nigel.wilson at sickkids.ca
Mon Oct 4 12:05:11 EDT 2010


Hi,

I am trying to get chromosome searches, e.g: chr1:12344..7890123,  to work within gbrowse2. My system is setup with 2 databases: hg18_scaffolds, and hg18_annotations. In hg18_scaffolds, I loaded the chromosome definitions from a gff3 file (e.g. chr1    hg18    chromosome  1   247249719   .   .   .   ID=chr1;Name=Chr1). In the hg18_annotations database, I loaded all the annotations that I require. The gff3 files for the annotations database do not contain any chromosome definitions. However when I do this, the search,  chr1:12344..7890123, returns an error: the landmark named chr1:12344..7890123 is not recognized. If I perform a different search, e.g. a clone name from the annotations database, this search will return the expected result. If I load everything (chromosome definitions, and annotations) into 1 database, both searches work properly.

Is there any way to keep my desired schema, that is, the 2 separate databases, and have searches such as chr1:12344..7890123 work?

Thanks,

Nigel Wilson
_________________________________________________
Bioinformatics Analyst
The Centre for Applied Genomics
The Hospital for Sick Children, MaRS Building - East Tower
*: 416.813.7032
*: 101 College St., Rm 14-701, Toronto, Ontario  M5G 1L7



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