[Feedback] Fwd: FW: promoter elements

[Peter Van Buren]

This request came to "info at warelab.org". Forwarding here to see if 
someone can answer.


-------- Forwarded Message --------
Subject: 	FW: promoter elements
Date: 	Thu, 19 Mar 2015 15:36:25 +0100
From: 	john at home <john.groenendijk at upcmail.nl>
To: 	info at warelab.org



Dear madam, sir,

  

Did you receive this correctly?

Thanks!

  

Van: john at home [mailto:john.groenendijk at upcmail.nl]
Verzonden: woensdag 18 maart 2015 15:03
Aan: 'ware at cshl.edu'
Onderwerp: promoter elements

  

Dear Dr. Ware,

  

We have an 1.8kb upstream region of a predicted gene in asparagus which we
would like to study further. One of our first questions would be to identify
cis-regulatory elements; the gene is expressed at low levels. Since we are
new to this and started a literature study, we came across your review
article of 2013 and some databases including plantprom.  I hope you can be
of assistance with identifying CRE predictions and/or directions on how we
should tackle this. Thanks in advance!

  

>upstream sequence including atg

GGGCCAACCCCGGACCGGGCCCGGACTGACGGGCTTTTTGGATTGGCCTAAACATATGCATTTATCTTTAAAAGTC
CATGTTATTTTTATGGGCTTGTTGCAAGCAAGCATGGCGGTAAACTAATCCTGGGTTTCAACATTAGTGCTAGTAA
AGCTTTGCCATCTTTTTTTTTTTTGCTTTAAAGGGCCAACCCCGGACCGGGCCCGGACTGACGGGCTTTTTGGATT
GGCCTAAACATATGCATTTATCTTTAAAAGTCCATGTTATTTTTATGGGCTTGTTGCAAGCAAGCATGGCGGTAAA
CTAATCCTGGGTTTCAACATTAGTGCTAGTAAAGCTTTGCCATCTTTTTTTTTTTTGCTTTAAAGTAATTTTTTAG
TAAACTTTTATTCTTAATAGTTCACAACAACACGAGAACCATACAAGTGAAAGAAATTTTATCCCCAAAAAATTTC
AAATAACCATCTCCAATTTTCACCAAATAATCCATCACAATTATCTATATTATACGCAATTCCACTGTTTTCTCGA
TTTTTTAACTTTACAAGTCATTAGATTCCTAGCACACAAAATTACCATTGTTTGTGGGAGGAAAAAAGGCATATTC
TACGCTTACAACCCAAGTCACACGATAATCACATTCTTCAAGCTTATGAGGAAACTTCGTCCTATTCCCCCAACTT
AAATTATTACAAAAAATCAGGAATAAGGAAAAAAGAAAAAATTTCCTTCTCTAAAATTTCAACTAAGACCATTATC
AACTTCTTCCAAATAATTTGTACCCATCATCAGTTTCTACCGTTCTCACTCATCGAAACATAAATTTTACAGTTTC
TACAAGAATAAAATAAACAAGACTGAGTCATAGAGAGAGGAGAAGATGGCAGCGAGATCGGAGGAGAAGATCTTGT
CGAACTAGTAGGGATGAGCTCAAGGTTGGTGGTGTTGGCACTGGGGGATGGGGAGAGATAGACGATGGGATTCGAC
GGAAAGATGAAGAGGGAGAGGAGGAGGGTTAGGAGAAAGTAGGAGAGGAGGTTGAATCGAAAGTTGAGAATGACGA
CGAGCTCGAGAAAGGAGTAGATGGCAGCGAGTTTGAAGGAGAAGATCTTGTCAAACAAGTTGAGCATGAGCTCAAG
GTCGGTAGTGGCGAAGGGCAAGGCGACAGAGTTAGACGGCAGATGCCAAGGAGAAGATGAAGAGGGAGAGGAGGAG
GCTAAGGAGAGAGTAGAAAGAGATGAGGCTGAATCACAAATGGGACGAAAGTTATGAGATCGAAGAGGGGTCTTTT
CGGAATTCTCAAACGACATTTCTTGGGGTAGAGTGAAAAGAGGGCTGAATCAAAAAGATGCAATGTATTTGGGGCT
AAAATACAAGAGGGAAATAATACGGTGGCTTGAGACACATCAAAGTTTAAATTTTAGGGCCTTCACACAACTTTCC
CAATTTTAAATTCTTGCTCTCCTAACCAAACGCAAAACAAAAGAAAAAAAACCGAAAAAAAAAGAAGAGAAAAATT
AATAACAGGAAGAGGCCGAAAGAGGCTTTTATTTTCCATTTCACTGGTGAGGGAGGAAGATTAACTGAACCTCCCC
TTTATACCCTCTAGTTATTCTAGCGACACGAACTCAACTCCAGGTGACAACATTCATAGAGACGATAAACCTCATC
ACCTCATATGCACCTTCACAACAATTACCATCTCCTGACAGCAAACCCGCCGGTCCCATATCAGCCCTCTGCATTC
GCCTCCCTTCGAGCTCCCTTCTCAAACCTGTCTTCTCCAAGTTCCAAGATTCACCTTCTTGAACAAGATTCAAGAA
CATG

  

Kind regards,

John Groenendijk

Wageningen University

The Netherlands




-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://brie4.cshl.edu/pipermail/feedback/attachments/20150320/b2436784/attachment.html>