[Feedback] [Website feedback] cannot clone some of trancripts

[Marcela K. Monaco]

Hi Yonghua,

The exon/intron structure for all transcripts is very similar.  I would
suggest that you look for supporting evidence (e.g., ESTs, cDNAs, RNA-Seq,
microarray data) that validate the existence of the transcripts that you
were unable to clone as they could also be annotation errors.

A direct sequence comparison can be seen in our browser:
http://ensembl.gramene.org/Zea_mays/Gene/TranscriptComparison?db=core;g=GRMZM2G122228;r=3:212913668-212919667;t=GRMZM2G122228_T02;t1=GRMZM2G122228_T01;t2=GRMZM2G122228_T03;t3=GRMZM2G122228_T02

Hope this helps,

Marcela

==
Marcela Karey Monaco, PhD
Gramene Project Coordinator
Cold Spring Harbor Laboratory
One Bungtown Road, Williams #5
Cold Spring Harbor, NY 11724
Phone: 516-367-8808
Fax: 516-367-6851


On Wed, Mar 26, 2014 at 8:34 PM, yonghuasun at 163.com <yonghuasun at 163.com>wrote:

> yonghua sun (yonghuasun at 163.com) sent a message using the contact form at
> http://gramene.org/contact.
>
> in maize genome, we cannot clone some of genes , ig, GRMZM2G122228_T03 and
> T02  transcripts through many primers, but we clone the GRMZM2G122228_T01
> transcript from the B73 cdna, can you give us some advice?  thanks
>
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