<div dir="ltr"><div><div>Hi Yonghua,<br><br>The exon/intron structure for all transcripts is very similar. I would suggest that you look for supporting evidence (e.g., ESTs, cDNAs, RNA-Seq, microarray data) that validate the existence of the transcripts that you were unable to clone as they could also be annotation errors. <br>
<br>A direct sequence comparison can be seen in our browser: <br><a href="http://ensembl.gramene.org/Zea_mays/Gene/TranscriptComparison?db=core;g=GRMZM2G122228;r=3:212913668-212919667;t=GRMZM2G122228_T02;t1=GRMZM2G122228_T01;t2=GRMZM2G122228_T03;t3=GRMZM2G122228_T02">http://ensembl.gramene.org/Zea_mays/Gene/TranscriptComparison?db=core;g=GRMZM2G122228;r=3:212913668-212919667;t=GRMZM2G122228_T02;t1=GRMZM2G122228_T01;t2=GRMZM2G122228_T03;t3=GRMZM2G122228_T02</a><br>
<br></div>Hope this helps,<br><br></div>Marcela<br></div><div class="gmail_extra"><br clear="all"><div><span style="border-collapse:collapse;color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px"><font size="1">==<br>
Marcela Karey Monaco, PhD</font> <font size="1"><br>Gramene Project Coordinator<br>Cold Spring Harbor Laboratory<br>One Bungtown Road, Williams #5<br>Cold Spring Harbor, NY 11724<br></font></span><span style="border-collapse:collapse;color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px"><font size="1">Phone: <a value="+15163676998" style="color:rgb(17,85,204)"><span>516</span>-<span>367</span>-<span><span style="background-image:initial;background-color:rgb(255,255,204);color:rgb(34,34,34);background-repeat:initial initial"><span><font size="1">8808</font></span></span></span></a><a value="+15163676998" style="color:rgb(17,85,204)"><span></span></a><br>
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<br><br><div class="gmail_quote">On Wed, Mar 26, 2014 at 8:34 PM, <a href="mailto:yonghuasun@163.com">yonghuasun@163.com</a> <span dir="ltr"><<a href="mailto:yonghuasun@163.com" target="_blank">yonghuasun@163.com</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">yonghua sun (<a href="mailto:yonghuasun@163.com">yonghuasun@163.com</a>) sent a message using the contact form at<br>
<a href="http://gramene.org/contact" target="_blank">http://gramene.org/contact</a>.<br>
<br>
in maize genome, we cannot clone some of genes , ig, GRMZM2G122228_T03 and<br>
T02 transcripts through many primers, but we clone the GRMZM2G122228_T01<br>
transcript from the B73 cdna, can you give us some advice? thanks<br>
<br>
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