Question GRO term

[katica at acoma.Stanford.EDU]

Dear Kikuchi,

Pankaj Jaiswal forwarded your request to POC. Currently, we only have term 
'cultured callus' and 'cultured embryo' in Plant structure ontology (PSO).
New term, embryogenic callus, would need to be introduced to PSO.

I have question for you: you described the callus as embryogenic, does 
this refer to callus that will give rise to somatic embryos, or to the callus 
induced from embryo tissue within seed, or both? I don't know enough about 
callus induction from rice seeds, some clarification would help with 
defining parent terms for the new term 'embryogenic callus'.

Also, there are no Growth stage terms for in vitro cultures. Since Plant 
Growth Stages are pertinent to a whole plant, we intentionally 
ommitted dealing with staging for tissue culture entities.

Best Regards,

Katica

> Shoshi Kikuchi wrote:
>> Dear Pankaj
>>
>> We are now preparing for the ontology-based description of our full-length
>> cDNA clones.
>>
>> I have a question. What is the appropriate description of embryogenic
>> callus. Many of our full-length cDNA libraries are originated from rice
>> callus induced from mature seed.
>>
>> 21 day-old callus after induction on the medium containing 2,4-D as auxin
>> from a mature seed.
>>
>> What kind of GRO term is the most appropriate one?
>>
>> Sincerely yours,
>>
>> Shoshi
>> ***********************************
>> Shoshi Kikuchi Ph.D
>> Plant Genome Research Unit
>> Division of Genome and Biodiversity Research
>> National Institute of Agrobilogical Sciences (NIAS)
>> Kan'non dai 2-1-2
>> Tsukuba Ibaraki 305-8602 Japan
>> TEL/FAX +81-29-838-7007
>> Email: skikuchi at nias.affrc.go.jp
>> WEB: ¡¡(RMOS) http://microarray.rice.dna.affrc.go.jp
>>           (KOME) http://cdna01.dna.affrc.go.jp/cDNA/
>> ************************************
>>
>>
>
>
>

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