New node in the Plant Structure Ontology

[Lincoln Stein]

Hi all,

I think we hashed through this at the spring meeting and the issue 
that came up is that there are cultured lines that don't correspond 
directly to any normal tissue.  The equivalent in human would be the 
HeLa cell line, which was derived (a long long time ago) from an 
ovarian cancer, but would hardly be described as "ovarian tissue" 
now.

Certainly for primary cultures an annotator might want to describe the 
source of a microarray tissue as "derived from primary culture of 
leaf tissue", where "leaf tissue" uses the appropriate PO term.  For 
established lines, though, I would be more comfortable descending 
from an "in vitro cell, tissue, organ culture" node.

Lincoln

On Tuesday 25 January 2005 01:58 pm, Pankaj Jaiswal wrote:
> I still do not think that we need to create a separate node "In
> vitro cell, tissue and organ culture". All the instances of this
> term can easily go under either cell/tissue/sprorophyte depending
> on the cultured plant structure.
>
> Also I would rephrase them as below, because one studies the
> expression of the genes or extracts enzymes/proteins/RNA from the
> actual cells or a tissue that were grown in an in-vitro culture,
> where the use of term "culture" is a recognition of method. The
> methodology can be described in the definition.
>
> leaf cell culture > cultured leaf cell
> root cell culture > cultured root cell
> protoplast culture > protoplast
>
> and appear in the tree as
>
> cell
> .i..cultured leaf cell
> .i..cultured root cell
> .i..protoplast
>
> tissue
> .i..callus-->I am sure it is an instance of meristem.
>
> embryo
> .i..somatic embryo
>
> -Pankaj
>
> Katica Ilic wrote:
> > Hi all,
> >
> > I finally put together In vitro (experimental) node for the Plant
> > Structure Ontology.
> >
> > Additional terms can be introduced later, these are the terms
> > needed for annotations in TAIR, Gramene and maizeGDB. I've had
> > second thoughts about terms that have 'culture' in the name, but
> > these are already existing terms, at least in TAIR and MaizeDGB.
> >
> > I would greatly appreciate your suggestions and comments,
> > especially regarding the definitions.
> >
> > The top node term is 'In vitro cell, tissue and organ culture'
> > and this term will go under the 'plant structure', as sibling to
> > the four top nodes:
> >
> > PO:0009002 : cell
> > PO:0009004 : gametophyte
> > PO:0009003 : sporophyte
> > PO:0009007 : tissue
> > New term: In vitro cell, tissue and organ culture
> > 	(i) protoplast culture
> > 	(i) cell culture
> > 		(i) leaf cell culture
> > 		(i) root cell culture
> > 	(i) callus
> > 		(i) callus culture
> > 	(i) embryo culture
> > 		(i) somatic embryo
> >
> >
> > Suggested definitions for the new terms:
> >
> > Term: in vitro cell, tissue and organ culture
> > Definition:
> > Experimentaly isolated and grown plant protoplast, cell, tissue
> > and organ, that involves, as a common factor, growth of
> > microbe-free plant material in an aseptic (sterile) environment,
> > such as sterilized nutrient medium in a test tube or petri dish.
> >
> > Term: protoplast culture
> > Def: In vitro isolation and maintenance of the cells from which
> > the entire cell wall has been enzymatically removed.
> >
> > Term: cell culture
> > Synonym: Cell suspension culture (cell suspension, carrot cell
> > suspension, tobacco cell suspension, maize cell suspension)
> > Def: In vitro grown isolated cells and small cell aggregates that
> > proliferate while suspended in liquid sterile medium or spread on
> > a solid agar medium.
> > Comment:  In cell cultures, the cells are no longer organized
> > into tissues.
> >
> > Term: leaf cell culture
> > Def: In vitro grown isolated cells and small cell aggregates that
> > originated from isolated leaf protoplasts (after they
> > re-established cell wall, or from callus that was induced from
> > leaf tissue).
> >
> > Term: root cell culture
> > Def: In vitro grown isolated cells and small cell aggregates that
> > originated from isolated protoplasts after they re-established
> > cell wall, or from callus that was induced from segment of root.
> >
> > Term: somatic embryo:
> > Def: An embryo arising from previously differentiated somatic
> > cells in vitro, rather than from fused haploid gametes, i.e.,
> > zygote.
> >
> > Term: Callus: An unorganized, proliferative mass of large,
> > thin-walled cells; a wound response.
> >
> > Term: Callus culture
> > Def: In vitro grown cell aggregates that originated from isolated
> > protoplasts, cell suspensions, or from callus that was induced in
> > sterile medium the supplemented by plant growth regulators.
> >
> > Term: embryo culture:
> > Def: In vitro development or maintenance of isolated mature or
> > immature zygotic embryos, or somatic embryos.
> >
> >
> > Katica
> >
> > -----------------------------------------------------------------
> >--------- Katica Ilic             		katica at acoma.stanford.edu
> > The Arabidopsis Information Resource 	Tel: (650) 325-1521 ext.
> > 253 Carnegie Institution of Washington 	FAX: (650) 325-6857
> > Department of Plant Biology 		URL: http://arabidopsis.org/
> > 260 Panama St.
> > Stanford, CA 94305
> > U.S.A.
> > -----------------------------------------------------------------
> >---------

-- 
Lincoln D. Stein
Cold Spring Harbor Laboratory
1 Bungtown Road
Cold Spring Harbor, NY 11724

NOTE: Please copy Sandra Michelsen <michelse at cshl.edu> on
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