<DIV>Dear Grains,</DIV> <DIV> </DIV> <DIV>I cloned LMW glutenin genes (of wheat) in pMOS-T blue and also pGEM-T cloning vectors, I confirmed positive clones using colony PCR, I used vector primers and also specific LMW glutenin primers. I got expected size amplicon. Then I incubated positive clones to isolate plasmids (alkaly lysis method) in large ammount for sequencing. But i am not getting the plasmids. </DIV> <DIV> </DIV> <DIV>Please let me know what is going wrong in my case. </DIV> <DIV> </DIV> <DIV>I appreciate your help in this regards.</DIV> <DIV> </DIV> <DIV>with best regards</DIV> <DIV>sincerely yours</DIV> <DIV> </DIV><BR><BR>If you judge people, you have no time to love them. -- Mother Teresa<br><br>Manoj D Oak Ph. D.<br>Scientist <br>Genetics Department<br>Plant Science Division <br>Agharkar Research Institute<br>Agarkar Road <br>Pune 411 004 <br>INDIA <br>Phone +91-20-25653680 (Off) <br>E-mail:
manojoak@yahoo.com<p> 
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