[Gramene] Thiamine biosynthesis

[Pankaj Jaiswal (OSU)]

Dear Aymeric,

Many thanks for your suggestions. I am forwarding them to the PotatoCyc 
developers from Cornell (copied). I will review the same for other 
plants especially cereal ones.

Best
Pankaj

-- 
Pankaj Jaiswal
Assistant Professor
Dept. of Botany and Plant Pathology
3082 Cordley Hall
Oregon State University
Corvallis, OR, 97331-2902, USA

Ph.: +1-541-737-8471
Fax: +1-541-737-3573
Web: http://jaiswallab.cgrb.oregonstate.edu/




On 3/6/2012 10:12 AM, Goyer, Aymeric wrote:
> Please provide your area of interest and pathway feedback
> Solanum tuberosum thiamine biosynthesis
> There are two errors in this pathway because the described pathway is
> based on data from E. coli that do not reflect what is happening in plants.
> First, it shows conversion of thiamine monophosphate to thiamine
> diphosphate, but this reaction does not occur in plants (for a Review
> see Goyer 2010 Phytochemistry 71, 1615-1624). Instead thiamine
> monophosphate is dephosphorylated to thiamine by a
> yet-to-be-characterized enzyme, then pyrophosphorylated by thiamine
> pyrophosphokinase (see Ajjawai et al 2007 Plant Mol Biol 65, 151-162).
> Second, 4-methyl-5-hydroxyethylthiazole phosphate is not synthesized
> from deoxy-xylulose-5-phosphate in plants but from NAD, glycine, and a
> sulfur donor (a cysteine located on the enzyme
> 4-methyl-5-hydroxyethylthiazole phosphate synthase – see Chatterjee et
> al 2011 Nature).
> Hope this helps.
> Aymeric
>
>
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