questions

[Junjian Ni]

Dear Zhiming,

What you need to do for next is, to use CMap (Maps database). I assume
after blast and checking the genome browser (contig view), you can
determine the position of the rice genome already. Then, you can go to
CMap:
http://www.gramene.org/cmap/

Using rice-matrix info,
http://www.gramene.org/db/cmap/matrix?show_matrix=1;map_set_acc=gt0306;link_map_set_acc=;

you can fine one maize genetical map that has high correspondence with the
rice sequence chromosome. You can alignment the maize genetic map together
with rice sequence map and then find the corresponding region on maize
genetic map, and finally get the molecular markers on the maize chromosome
on the region.

For more details, please follow our tutorials of Maps to know more about
how to use it.
http://www.gramene.org/tutorials/cmap.html

Let's know if having further questions and thanks for being interested in
using Gramene db.

Junjian Ni

> Dear Dr.
>    I am a PH D from the maize research institute of sichuan agricultural
> university in china. I have gained some ESTs from a Maize subtractive
> library, including some maize and rice ESTs.   I want to map it on a
> rice chromosome, and find the nearst molecular Markers in maize
> chromosome. I know I should use the Blast to find the sequence
> alignment and contig view of the rice genome. But I do not know the
> next what I can do. Could you give me some adivice and help me to do
> this work!
>   I look forward hearing from you!
>
>   Best wishes!
>
>   Zhiming Zhang
>   zhangzm2 at yahoo.com.cn
>
>
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Junjian Ni
Gramene Curator
G15, Bradfield Hall,
Cornell University,
Ithaca, NY 14853