Finger printing using RM primer

[Rajkumar]

Dear Dey,


Well. In the same way how you score for RAPD. But it depends on the purpose,
for which you use the data.

If you have intended to calculate genetic distance and construct a phenetic
tree, then you would just require converting your data into binary based on
presence or absence. But if you would like to study the population
structure, you may need to score as homo/heterozygous and further convert to
respective numerics depending on the software you choose to analyse the
data.

SSLP is NOT a multi-locus marker and you will ofcourse 1 or 2 bands (in a
diploid species).

Regards,

Rajkumar

_______________________________________
From: owner-gramene at gramene.org [mailto:owner-gramene at gramene.org] On Behalf
Of narottam dey
Sent: Monday, August 16, 2004 7:00 AM
To: gramene at gramene.org
Subject: Finger printing using RM primer

Respected all,
       I am Narottam Dey,senion research fellow Bose Institute, Kolkata,
India.
Would you tell me if possible the way by which  I can prepare the 1/0 matrix
by using Rice microsatellite markers in DNA fingerprinting of germplasm. As
in RAPD for a particular primer we get a number of bands but in case SSLP I
get only one specific band.
Thamking you very much for your attantion.
With regards,
Narottam
 
Narottam Dey.
Senior Research Fellow.
Dept Of Botany
Bose Institute.
93/1,APC road,
Kolkata-700009,(W.B)
India.
Ph-91-33-23679670 (R),23506619-ext-330(o)