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[Sabu]

Hello,

My question is on the selection of microsatellite
markers for QTL mapping in rice. We would like to
analyse, say 150 RM markers and from the available
list of 600 markers in the gramene site
(http://www.gramene.org/microsat/RM_primers.html)
which ones we can select? What are the criteria? There
are things like Distance on chromosome, Repeat type
and length, No. of alleles, PIC, PCR product in, Size
range, Anneal temp (ºC) etc. Can you please comment on
this problem.

Thanks.. Sabu


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