Query

[Priya Ranjan]

 Respected Sir/Madam
    I have a query and I would be grateful for your help. I have some
10000 EST sequences and I want to match EST sequences with the database
(say nr at NCBI)using blast and give some functional annotation to the
sequences.

I can go by following methods
1)I can take each sequence. Blast it against nr database and then provide
the functional annotation based on the blast result. But that would really
be a time consuming process

2) After some literature survey I found out that this may be another
process.From the 10000 sequences I can remove the redundncy by using the
staden package or a similar assembler. Then when I have the non redundant
set I can search it agaianst the database and give functional annotation.
Still this would take a lot of time (Say there are 2000 non redundant
sequences)


I hope I have given the background of the query. I dont know whether you
did similar type of work but if  you can suggest me some other  ways to
get the functional annotation for ESTs, I would be very grateful.                                    


regards
Priya Ranjan