I'm working with Dr. Mary Dolan from Jackson Laboratory in Maine. We have built a Chado database and loaded all of the BioPerl modules. In following the instructions to load a GFF file (<a href="http://gmod.org/wiki/Load_GFF_Into_Chado">http://gmod.org/wiki/Load_GFF_Into_Chado</a>) I ran into a snag. I first tried loading the example yeast features on that page, which Mary used as the example for building our mouse GFF file, and got the following error:<br>
<br>gene@genosis:~/gmod-1.0/yeast_data$ gmod_bulk_load_gff3.pl --organism yeast --gfffile saccharomyces_cerevisiae.gff.sorted<br>Preparing data for inserting into the genosis_chado database<br>(This may take a while ...)<br>
Operation "ne": no method found,<br> left argument in overloaded package Bio::Annotation::SimpleValue,<br> right argument has no overloaded magic at /usr/local/bin/gmod_bulk_load_gff3.pl line 811, <GEN0> line 1.<br>
<br>Is there just something malformed in our GFF file, or have I misused the bulk load script?<br><br>Thanks for your help!<br>Jake<br><br>Jake Emerson<br>Spatial Information Science and Engineering<br>University of Maine<br>
127 Boardman Hall<br>Orono, ME 04469<br>