[Gmod-help] Gbrowse2 semantic feature switching
Noah Fahlgren
fahlgren at cgrb.oregonstate.edu
Fri Jun 4 20:04:56 EDT 2010
I have a database populated with sequenced small RNA and I am trying to
create a track that displays the individual sequences below 1,000 nts
and displays histogram density data with scrolling windows above. Here
is my track configuration:
[lib127]
feature = smallRNA127
database = smallRNA
glyph = generic
key = Col-0
height = 4
fgcolor = \&multicolorDisplay
bgcolor = \&multicolorDisplay
strand_arrow = 1
description = 0
label = sub {
my $feature = shift;
my $note = $feature->score." RPM";
return $note;
}
label_density = 50
bump density = 1000
category = Fahlgren et al. 2009
citation = small RNA from wild type (Col-0) whole-aerial, bolting
plants (Fahlgren et al. 2009).
[lib127:1000]
feature = smallRNA127_100
database = histograms
glyph = xyplot
height = 50
fgcolor = black
bgcolor = black
strand_arrow = 0
description = 0
label = 0
group_on = display_name
graph_type = boxes
max_score = 100
min_score = -100
clip = 1
part_color = \&multicolorDisplay
When I zoom out to 1,000 nts or more I get individual xyplot graphs for
each datapoint instead of a continuous graph. If I make the xyplot
stanza the default feature then I get normal xyplots. Here is a sample
of what I loaded into our Bio::DB::SeqFeature MySQL database:
Individual small RNA:
Chr1 ASRP smallRNA127 75 98 1.52 - .
ID=ASRP1672607;Index=0
Chr1 ASRP smallRNA127 154 177 1.52 + .
ID=ASRP782716;Index=0
Chr1 ASRP smallRNA127 170 192 4.56 + .
ID=ASRP331607;Index=0
Chr1 ASRP smallRNA127 171 194 1.52 + .
ID=ASRP7739156;Index=0
Chr1 ASRP smallRNA127 543 566 1.52 + .
ID=ASRP2341059;Index=0
Chr1 ASRP smallRNA127 594 617 1.52 + .
ID=ASRP3944461;Index=0
Chr1 ASRP smallRNA127 752 775 1.52 + .
ID=ASRP2891574;Index=0
Chr1 ASRP smallRNA127 772 795 1.52 + .
ID=ASRP827103;Index=0
Histogram data:
Chr1 ASRP smallRNA127_100 110 130 1.52 + .
Name=smallRNA127_100;SizeClass=24;Index=0
Chr1 ASRP smallRNA127_100 130 150 7.6 + .
Name=smallRNA127_100;SizeClass=23;Index=0
Chr1 ASRP smallRNA127_100 150 170 7.6 + .
Name=smallRNA127_100;SizeClass=23;Index=0
Chr1 ASRP smallRNA127_100 170 190 7.6 + .
Name=smallRNA127_100;SizeClass=23;Index=0
Chr1 ASRP smallRNA127_100 190 210 7.6 + .
Name=smallRNA127_100;SizeClass=23;Index=0
Chr1 ASRP smallRNA127_100 210 230 6.08 + .
Name=smallRNA127_100;SizeClass=23;Index=0
Chr1 ASRP smallRNA127_100 510 530 1.52 + .
Name=smallRNA127_100;SizeClass=24;Index=0
Chr1 ASRP smallRNA127_100 530 550 1.52 + .
Name=smallRNA127_100;SizeClass=24;Index=0
Chr1 ASRP smallRNA127_100 550 570 3.04 + .
Name=smallRNA127_100;SizeClass=24;Index=0
Am I missing something? I used to do this in Gbrowse 1.7, but maybe
something has changed.
Thanks,
Noah
--
Noah Fahlgren
Graduate Research Assistant
Center for Genome Research and Biocomputing
3021 ALS Building
Oregon State University
Corvallis, OR 97331
Phone: 541-737-3679
Email: fahlgren at cgrb.oregonstate.edu
More information about the Gmod-help
mailing list