[Gmod-help] Chado - Apollo Tier Display

Yap, Soon syap at jcvi.org
Wed Jul 8 17:48:00 EDT 2009


Dear support,

 

  I'm trying to use Apollo for annotation purposes. I've setup the Chado
database and was able to load in the fasta file into the database. Other
then the fasta file, I was able to load some of the gene prediction gff
file to the chado database in gff3 format with column no. 3 being CDS,
gene, exon, mRNA, and cDNA_match. 

 

  I'm able to use Artemis to connect to the database, and able to
visualize the feature I loaded in. However, when I use Apollo to view
the database, all of the above mention features cannot be seen on the
Apollo, even I've tried to expand all the tiers. I know  need to setup
the tiers style to be able to view the features. Under the preferences,
Type -> Tier, I've created a new tier profile with Visible, Labeled and
Expanded option ticked. Under the drawing group, I've also created a new
drawing group, and I've put in CDS, gene, exon, mRNA, and cDNA_match in
the "Apply to following data types" window. I've save the tiers file,
restart Apollo, but I'm still unable to see the features I've loaded in.


 

  Any guide to solve this problem is highly appreciated, thank you!

 

  Regards,

 

SoonJoo,Yap

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