[Gmod-help] GBrowse Quantitative data upload

[Tripti Grover]

Hi
Thanks Dave for your reply.


I have uploaded Quantitative data for three experiments on my Mac OS X  by
creating their wig files and then uploading the respective  gff3 files
created after running the wiggle2gff3 script. Using the glyph wiggle_density
i can observe transcriptional profiles for all three experiments viz
seedling, root, young leaf (YL) as separate tracks but they are all grouped
under one checkbox heading "Transcriptional Profile". I need separate
checkbox for each experiment so that the user can turn a track on or off as
desired.



For this i created separate track configuration stanzas like [Transchip 1],
[Transchip 2], ...etc in the conf file as shown below ,but,found it of no
use as,  checking one box shows expression profile for all three
experiments. Like if root is checked it shows data about all stages
seedling, root as well as young leaf.


[TransChip1]

feature        = microarray_oligo

glyph          = wiggle_density

height         = 30

bgcolor        = blue

category       = Genes

description    = 1

key            = Seedling


[TransChip2]

feature        = microarray_oligo

glyph          = wiggle_density

height         = 30

bgcolor        = blue

category       = Genes

description    = 1

key            = Root


[TransChip3]

feature        = microarray_oligo

glyph          = wiggle_density

height         = 30

bgcolor        = blue

category       = Genes

description    = 1

key            = Young Leaf


Secondly, clicking the wiggle density glyph at a particular position should
display the corresponding score which is not getting displayed. It displays
exactly same information at all positions, even the length and position
values displayed are always the same.I have attached 2 screen shots for both
my problems.

Kindly suggest the modifications i need to make so that only the desired
track is displayed and score details can also be viewed . The data has to be
uploaded using a wig file, and i have around 20 experiments to be displayed.


Thanking You


Regards
Tripti

On Thu, Aug 13, 2009 at 12:39 PM, Dave Clements, GMOD Help Desk <
gmodhelp at googlemail.com> wrote:

> Hi Tripti,
>
> First I apologize for the delayed response.  Lincoln's been on
> holiday, and Scott and I were at GMOD Europe.  I just got back in the
> states yesterday.
>
> And, in case you haven't already got this one figured out, ...
>
> I understand some of what is going on here, but not all of it.  Part
> of the problem is that you need a way to distinguish between the two
> types of data.  Both the Seedling and Root data have the same source
> (GFF3 column 2) and type (column 3) values.  The "feature" clause is
> what determines what data is shown in which track.  To get the
> different data in different tracks try adding "root" and "seedling" to
> the source column. For example:
>
> Chr1 Seedling_Os01g01010 microarray_exp 1903 9817 5.758830 . .
> Name=Seedling
>
> Then change the track definition to
>
> [TransChip1]
> feature        = microarray_exp:Seedling_Os01g01010
> ...
>
> [TransChip2]
> feature        = microarray_exp:Root_Os01g01010
> ...
>
> I think this will get you the two types of data showing up in the
> different tracks.  Also, I don't think "microarray_exp" is an official
> SO term.  Is "microarray_oligo" what you want?
> http://www.sequenceontology.org/miso/current_cvs/term/SO:0000328
>
> The part I don't understand is why you are not currently seeing both
> the seedling and root data in both tracks.
>
> Hope this helps,
>
> Dave C
>
> On Sun, Aug 2, 2009 at 10:34 PM, Tripti Grover<tripti.grover at gmail.com>
> wrote:
> > Dear All,
> > We have been trying to upload Quantitative Data for more than one
> experiment
> > to GBrowse locally installed on Mac OS X. In order to do this we've been
> > following the Administrative tutorial according to which gff3 file
> contains
> > the data to be displayed.
> > Ex :
> > Chr1 LOC_Os01g01010 microarray_exp 1903 9817 5.758830 . . Name=Seedling
> > Likewise we've appended the above file with the data of another
> experiment,
> > the sample data for which is like :
> > Chr1 LOC_Os01g01010 microarray_exp 1903 9817 14.578833 . . Name=Root
> > The modifications done in the conf file are as follows :
> > [TransChip1]
> > feature        = microarray_exp
> > glyph          = graded_segments
> > graph_type     = boxes
> > fgcolor        = red
> > bgcolor        = orange
> > height         = 10
> > min_score      = 0
> > max_score      = 15
> > category       = Transcriptional Profile
> > key            = Seedling
> > [TransChip2]
> > feature        = microarray_exp
> > glyph          = graded_segments
> > graph_type     = boxes
> > fgcolor        = red
> > bgcolor        = orange
> > height         = 10
> > min_score      = 0
> > max_score      = 15
> > category       = Transcriptional Profile
> > key            = Root
> >
> > With these data and conf files the display we get in GBrowse is both for
> >  Seedling and Root but,the color gradation displayed is the same for both
> > even though the scores provided in the gff3 files differ a lot.Probably,
> the
> > data is being displayed from the first data set and not from the other. I
> am
> > also attaching the screen shot of GBrowse interface for the display we
> are
> > getting.
> > Kindly suggest what modifications we need to do in Conf and GFF3 files so
> > that different data sets can be displayed with corresponding color
> > gradations.
> > Thanking You
> > Regards
> > Tripti
> > Junior Research Fellow
> > Department of Plant Molecular Biology
> > University of Delhi
> > India
>
>
>
> --
> * Please keep responses on the list!
> * Was this helpful?  Let us know at
> http://gmod.org/wiki/Help_Desk_Feedback
>
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