[Gmod-gbrowse] [Gmod-help] Re: [Gmod-schema] Error loading GFF3 file into chado

[Scott Cain]

Hi Shu,

The file in question:

ftp://ftp.jgi-psf.org/pub/JGI_data/Sorghum_bicolor/v1.0/Sorbi1_GeneModels_Sbi1_4_Sbi1_4.gff.gz

is definitely not GFF3.  The first couple of lines look like this:

chr_1   JGI     exon    2164    2829    .       +       .       name  
"Sb01g000200"; transcriptId 5257096
chr_1   JGI     CDS     2164    2829    .       +       0       name  
"Sb01g000200"; proteinId 5027909; exonNumber 1

The 9th column needs to be in "tag=value" format and "name" should be  
capitalized, and it would be nice to have gene and mRNA features to go  
with the exon and CDS features, rather than depending on inferring  
their existance (the Chado loader will fail to do that, and the  
Bio::DB::SeqFeature::Store will probably be successful, but getting  
GBrowse to display it properly will likely not be easy).

Scott


On Aug 14, 2009, at 4:35 PM, Shengqiang Shu wrote:

> This file is in format of GFF3, Is this loading problem due to file  
> not conforming to GFF3 standard? In term of SO terms, it uses gene,  
> mRNA, exon, CDS. No problem, is there?
>
> Only thing looks like missing is Name attribute. Is Name attribute  
> required? From scanning through doc on sequence ontology site, it  
> did not say Name is required.
>
> I know this file is produced by one of our members. If you guys  
> point out what is the problem with file in term of conforming to  
> GFF3 standard, it would be very helpful.
>
> By the way, FASTA in GFF3 is optional, isn't? Don't know why loader  
> can not handle it? ah, the file is annotation GFF3 file, it does not  
> contain genomic features. Is this causing the loading problem?
>
>
> Shu
>
>
> On Aug 14, 2009, at 12:54 PM, Chris Fields wrote:
>
>> Yikes, another GFF2 variant?
>>
>> chris
>>
>> On Aug 14, 2009, at 2:50 PM, Jason Stajich wrote:
>>
>>> I also have these script jg_gff2gff3.pl in gff_tools of http://github.com/hyphaltip/genome-scripts/tree/master
>>> and
>>> gff3_to_JGIgff.pl for converting back into JGI gff from GFF3.
>>>
>>> -jason
>>>
>>> On Aug 14, 2009, at 11:01 AM, Don Gilbert wrote:
>>>
>>>>
>>>>> Has anyone out there written a JGI GFF2 to GFF3 parser
>>>>
>>>> I've written both ways: jgi to gff3, and gff3 to jgi-gff (which  
>>>> is a
>>>> variant of gff2).
>>>> Find here
>>>> http://wfleabase.org/release1/current_release/supplement/jgi_gff/
>>>> as jgi2gff.pl  (and dpulex_gff2jgi.pl  for the other way)
>>>>
>>>> This script will want some revision for your data, and the variant
>>>> of JGI's gff
>>>> I worked with may differ from yours.  Look at the sample at the top
>>>> of the
>>>> script to see if it matches yours.
>>>>
>>>> - Don
>>>>
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>>> --
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>>> jason.stajich at gmail.com
>>> jason at bioperl.org
>>>
>>>
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-----------------------------------------------------------------------
Scott Cain, Ph. D. scott at scottcain dot net
GMOD Coordinator (http://gmod.org/) 216-392-3087
Ontario Institute for Cancer Research