[Feedback] Site Feedback: two sequences for RM1359

[feedback at gramene.org]

URL         : http://archive.gramene.org/markers/microsat/all-ssr.html

Subject     : Site Feedback: two sequences for RM1359

Name        : Gretchen Zaunbrecher

Email       : gzaunbrecher at agcenter.lsu.edu

Organization: LSU AgCenter Rice Research Station

Comments    : 

I found two sequences for RM1359 and was hoping you could tell me which is correct.  I have found each under the following addresses:

http://archive.gramene.org/markers/microsat/ssr.html

Locus Name	Primer Name	Forward Primer	Reverse Primer
RM1359	MRG0359.IRRI0359	CTCGCGAGGAAGAAGACAAC	CGCCGGCTGGTTAATTAATC

This is sequence I have found in a couple articles.  I have used these primers in PCR with success.


http://archive.gramene.org/markers/microsat/all-ssr.html


marker_id	marker_name	marker_type	species	primer_forward	primer_reverse	repeat_motif	anneal_temperature	product_size	sequence_source	linkage_group

24987257	RM1359	SSR	Oryza sativa Japonica Group	AACGAATTCTATTTTGCGTC	TTCTTCTCATTTCAATTCGC	(AG)25	55	170	AY018034


This sequence I have never used.  


Thanks

http://www.warelab.org/bugs/view.php?id=5529